Vessel for formation
of bilayer lipid membranes by
Mueller-Rudin method*
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Last modified: January 1, 2006
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- body of the vessel is made from Teflon
- size 60x30x30 mm
- volume of each chamber10 cm3
- thickness of the septum between chambers 0.3 mm
- diameter of the hole between chambers 1 mm
- windows are made from glass
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Crossing trough the
septum separeted two chambers in
the
measurement
vessel.
The bilayer lipid membrane
is formed inside the hole in
the septum.
The membrane is surrounded by the Plateau-Gibbs border. The border is
composited
of a membrane forming solution (polar lipids dissolved in an organic
solvent,
e.g. decane).
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* Mueller P., Rudin D., Tien H.T., and Wescott W.C.,
Reconstitution
of excitable cell membrane structure in vitro, Circulation,
26
(1962) 1167-1171.
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