Bilayer lipid
membranes
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Last modified: January 1, 2006
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Investigation of membrane properties and
phenomena:
- electrical properties
- voltage-dependent capacitance
- voltage-dependent changes of membrane structure
- transport through the membranes
- development of measurement methods
- electroporation
- mechanisms of BLM formation process
- electrochemical sensors, biosensors, biomimetic sensors
Our investigations, methods and experience:
 Membrane capacitance during BLM formation
process by Mueller-Rudin method
Photos of bilayer lipid membranes
formed by Mueller-Rudin method
Bolaamphiphilic steroid dimers
The vessel for formation of
the
membranes
The device for electrical
measurements
The optical setup
Electrical
meausurement of membrane thickness
Electroporation
of the BLMs in current-clamp conditions
Method for
measurement of membrane resistance
The following electrical measurement
methods for
membrane studies were used:
- Recording of membrane capacitance - studies of membrane
formation
process,
measurments of membrane thickness, studies of membrane thickness
changes
caused by membrane potential.
- Cyclic voltammetry - measurement of membrane resistance,
investigation
of transport processes.
- Chronoamperometry - investigation of transport processes.
- Capacitance-voltage characteristics - investigation of
surface
potentials.
- Chronopotentiometry - studies of mechanism of
electroporation
phenomenon
- generation of single pore in membrane.
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