Jesteś tutaj

Dr hab. Jacek Wierzchowski, prof. UWM

 

dr hab. Jacek Wierzchowski, prof. UWM

pokój 103

tel: +(48)089 523 33 24

e-mail: jacek.wie@uwm.edu.pl

 

 

Curriculum Vitae

Born:

1951 in Warsaw, Poland

Employment:

Department of Biophysics, University of Warmia & Mazury in Olsztyn, 4 Oczapowskiego St., 10-721 Olsztyn, Poland

Associate Professor of Biophysics since 1.07.2003 r.

Education & Degrees:

1976 r., M. Sc. in Biophysics, Warsaw University

1981 r., Ph. D. Warsaw University, Dep Biophysics; supervisor: Professor David Shugar

1998, Habilitation in Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences.

Former employment:

1976-80, Warsaw University, Department of Biophysics, postgraduete student. 

1980-1988, Agricultural University in Poznan, Poland, Department of Physics, assistant professor.

1988-1997, Warsaw Medical School, Faculty of Pharmacy, Department of Physical Chemistry, assistant professor.

1998-2003, University of Podlasie in Siedlce, Poland, Institute of Chemistry, associate professor.

Overseas practice:

1984-1985, 12 months, Department of Chemistry, Dalhousie University, Halifax, (Canada), research fellow

1985-1987 i 1991 (3 years), Center for Biochemical & Biophysical Research and Medicine, Harvard Medical School, Boston, MA (U.S.A.), research fellow and lecturer.

Membership:

Polish Biophysical Society

International Society for Nucleosides, Nucleotides and Nucleic Acids (ISN3A);

Research scope:

Fluorescence spectroscopy and its applications in enzymology, fluorescent purine analogs and their interaction with enzymes of purine metabolism, analytical biochemistry, enzymes of alcohol metabolism, applications of to medical diagnostics.

 

Selected papers

1.  WIERZCHOWSKI J., Kuśmierek J, Giziewicz J, Salvi D, Shugar D., 1980, Acta Biochim. Pol. 27,  35-56:  Analogues of formycin A and B: Synthesis and some properties of methyl derivatives of 7-amino- and 7-ketopyrazolo[4,3-d]pyrimidines.

2.  WIERZCHOWSKI J., Szczęśniak M, Shugar D., 1980, Z. Naturforsch. 35c, 878-889:  Fluorescence emission properties of the cation of 4-aminopyrazolo[3,4-d]pyrimidine, an adenine analogue: evidence for phototautomerism.

3.  WIERZCHOWSKI J., Shugar D., 1982,  Photochem. Photobiol. 35,  445-458,  Luminescence studies on formycin, its aglycone, and their N-methyl derivatives: tautomerism, sites of protonation and phototautomerism.

4.  WIERZCHOWSKI J., Shugar D.,  1983,  Z. Naturforsch. 38c,  67-73,  Sensitive fluorimetric assay for adenosine deaminase with formycin as substrate and substrate and inhibitor properties of some pyrazolopyrimidine and related analogues.

5.  Elbanowski M., WIERZCHOWSKI J., Paetz M., Sławinski J.,  1983,  Z. Naturforsch. 38a,  808-810:  Chemiluminescent oxidation reaction of europium (2+) ions with hydrogen peroxide.

6.  WIERZCHOWSKI J., Lassota P., Shugar D.,  1984,  Biochim. Biophys. Acta  786,  170-178:  Continuous fluorimetric assay of 5'-nucleotidase with 5'-formycin phosphate as substrate, and its application to properties of substrates and inhibitors.

7.  WIERZCHOWSKI J., Sierakowska H., Shugar D.,  1985,  Biochim. Biophys. Acta 828,  109-115:  Continuous fluorimetric assay of nucleotide pyrophosphatase: kinetics, inhibitors, and extension to dinucleoside oligophosphatases.

8.  Kulikowska E., Bzowska A., WIERZCHOWSKI J., Shugar D.,  1986,  Biochim. Biophys. Acta  874,  355-366:  Properties of two unusual, and fluorescent substrates of purine-nucleoside phosphorylase: 7-methylguanosine and 7-methylinosine.

9.  WIERZCHOWSKI J., Sławinska D., Sławinski J.,  1986,  Z. Phys. Chem. N.F. 148,  197-214,  Carbonate and superoxide ion-radicals as intermediates in the chemiluminescence of the ferricyanide-hydrogen peroxide redox system.

10.  Arnold D.R., Fahie B.J., Lamont L.J., WIERZCHOWSKI J., Young K.M., 1987, Can. J. Chem. 65, 2734-2743:  Radical ions in photochemistry. 19. 1,n-radical ions. The photosensitized (electron transfer) formation of 1,5-radical cations.

11. WIERZCHOWSKI J., Dafeldaecker W.P., Holmquist B., Vallee B.L., 1989, Anal. Biochem. 178,  57-62:  Fluorometric assays for isozymes of human alcohol dehydrogenase.

12. WIERZCHOWSKI J., Holmquist B., Vallee B.L., 1992, Anal. Chem. 65 181-187: Determination of human serum alcohol dehydrogenase using isozyme-specific fluorescent substrates.

13. WIERZCHOWSKI J., Interewicz E., Wroczynski P., Orlanska I., 1996, Anal. Chim. Acta 319, 209-219: Continuous fluorimetric assay for human aldehyde dehydrogenase and its application to blood analysis.

14. WIERZCHOWSKI J., Wielgus-Kutrowska B., Shugar D., 1996, Biochim. Biophys. Acta 1290, 9-17: Fluorescence emission properties of 8-Azapurines and their nucleosides, and application to the kinetics of the reverse synthetic reaction of purine nucleoside phosphorylase.

15. WIERZCHOWSKI J., Wroczynski P., Interewicz E., 1996, Acta Pol. Pharm. Drug Res. 53, 203-208: Selective assay of the cytosolic forms of the aldehyde dehydrogenase in rat, with possible significance for investigations of cyclophosphamide cytotoxicity.

16.  WIERZCHOWSKI J., Wroczynski P., Interewicz E., Orlanska I., Przybylski J., 1996, Fluorogenic substrates reveal genetic differences in aldehyde-oxidating enzyme patterns in rats, in "Fluorescence Microscopy and Fluorescent Probes", J. Slavik, edytor, Plenum Publishing Corp., New York, pp. 227-231.

17. Wielgus-Kutrowska B., Kulikowska E., WIERZCHOWSKI J., Bzowska A., Shugar D., 1997: Nicotinamide riboside - an unusual, non-typical substrate of purified purine nucleoside phosphorylases.  Eur. J. Biochem 243, 408-414.

18. WIERZCHOWSKI J., Wroczynski P., Laszuk K., Interewicz E., 1997, Fluorimetric detection of aldehyde dehydrogenase activity in human blood, saliva and organ biopsies, and kinetic differentiation between class I and class III isozymes, Analytical Biochemistry 245, 68-79.

19. Wroczynski P., Barut-Cichocka J., WIERZCHOWSKI J., Szubert A., Polański J., 1997, Fluorimetric assay for aldehyde dehydrogenase activity in tissue - some biomedical applications, in "Fluorescence Microscopy and Fluorescent Probes, 2.", J. Slavik, editor, Plenum Publishing Corp., New York., pp. 209-213.

20. Wroczynski P., Laskowska A., WIERZCHOWSKI J., Szubert A., Polański J., Słowiaczek M., (1998).  Aldehyde dehydrogenase activity in tumours - assay with potential prognostic value for cyclophosphamide chemotherapy. Acta Biochim. Polon. 45, 33-45.

21. WIERZCHOWSKI J., E. Kulikowska, A. Bzowska, A. Holy, L. Magnowska and D. Shugar, 1999, Interactions of purine nucleoside phosphorylase with antiviral acyclic nucleoside phosphonate inhibitors – kinetics and emission studies. Nucleosides & Nucleotides, 18, 875-6.

22. Wielgus-Kutrowska B., J. Tebbe, J. WIERZCHOWSKI, D. Shugar, W. Saenger, G. Koellner and A. Bzowska, 1999, Binding of substrates by purine nucleoside phosphorylase (PNP) from Cellulomonas sp. – kinetic and spectrofluorimetric studies. Nucleosides & Nucleotides, 18, 871-872.

23. Bojarska E., R. Kraciuk, J. WIERZCHOWSKI, Z. Wieczorek, J. Stępiński, M. Jankowska, E. Starzyńska, A. Guranowski, E. Darżynkiewicz, 1999, Hydrolysis of some mRNA 5’-CAP analogs catalyzed by the human Fhit protein and Lupin ApppA hydrolases. Nucleosides & Nucleotides, 18, 1125-6.

24. Kierdaszuk B., A. Modrak-Wójcik, J. WIERZCHOWSKI, D. Shugar, 2000, Formycin A and its N-methyl analogues, specific inhibitors of E. coli purine nucleoside phosphorylase: induced tautomeric shift on binding to enzyme, and enzyme ® ligand fluorescence resonance energy transfer, Biochim. Biophys. Acta 1476, 109-128.

25. Wroczynski P., WIERZCHOWSKI, J., 2000, Aromatic aldehydes as fluorogenic indicators for human aldehyde dehydrogenases and oxidases: substrate and isozyme selectivity. Analyst, 125, 511-516.

26. WIERZCHOWSKI J., M. Ogiela, B. Iwańska and D. Shugar, 2002, Selective fluorescent and fluorogenic substrates for purine-nucleoside phosphorylases from various sources, and direct fluorimetric determination of enzyme levels in human and animal blood.  Analytica Chimica Acta 472, 63-74.

27. Wroczyński P., J. WIERZCHOWSKI, J. Gołąb, M. Błażejczyk, D. Borecka, 2002, Determination of aldehyde dehydrogenase (ALDH) isozymes in human cancer samples - comparison of kinetic and immunochemical assays.  Molecules 7, 896-901 (www.mdpi.org/molecules).

28. WIERZCHOWSKI J., A. Bzowska, K. Stępniak and D. Shugar, 2004, Interactions of Calf Spleen Purine Nucleoside Phosphorylase with 8-Azaguanine, and a Bisubstrate Analogue Inhibitor: Implications for the Reaction Mechanism. Z. Naturforsch. 59c, 713-725.

29. J. WIERZCHOWSKI, K. Stępniak, A. Bzowska and D. Shugar, 2005, Spectroscopic and kinetic studies of interactions of calf spleen purine nucleoside phosphorylase with 8-azaguanine and its 9-(2-phosphonylmethoxyethyl) derivative, Nucleosides, Nucleotides & Nucl. Acids 24, 459-464.

30. M. Mojzych, A. Rykowski and J. WIERZCHOWSKI, 2005, Pyrazolo[4,3-e][1,2,4]triazines: purine analogues with electronic absorption in the visible region.  Molecules 10, 1298-1306. (www.mdpi.org/molecules).

31. P. Wroczyński, M. Nowak, J. WIERZCHOWSKI, A. Szubert J. Polański, 2005, Activities of cytosolic aldehyde dehydrogenase isozymes in colon cancer: determination using selective, fluorimetric assays. Acta Pol. Pharm. Drug Res 62, 427-433.

32. J. WIERZCHOWSKI, J. Sepiol, D. Sulikowski, B. Kierdaszuk, D. Shugar, 2006,  Fluorescence emission properties of 8-azaxanthine and its N-alkyl derivatives: Excited-state proton transfer and potential applications in enzymology. J. Photochem. Photobiol. A., 179, 276-282.

33.  P. Wroczyński, J. Giebułtowicz, J. Piekarczyk, J. WIERZCHOWSKI, 2006, Fluorimetric Detection of Aldehyde Dehydrogenase Activity In Human Saliva in Diagnostic of Cancers of Oral Cavity. Acta Pol. Pharm. Drug Res63, 407-409.

34. K. Stępniak, B. Żiruić, J. WIERZCHOWSKI, A. Bzowska, 2007, Fluorescence studies of calf spleen purine nucleoside phosphorylase (PNP) complexes with guanine and 9-deazaguanine. Nucleosides, Nucleotides & Nucl. Acids 26, 841-847.

35. J. WIERZCHOWSKI, M. Pietrzak, J. Stepinski, J. Jemielity, M. Kalek, E. Bojarska, M. Jankowska-Anyszka, R.E. Davis, E. Darzynkiewicz, 2007, Kinetics of C. elegans DcpS cap hydrolysis studied by fluorescence spectroscopy. Nucleosides, Nucleotides & Nucl. Acids 26, 1211-1215.

36. WIERZCHOWSKI J., M. Pietrzak, M. Szeląg, P. Wroczyński, 2008, Salivary aldehyde dehydrogenase – reversible oxidation of the enzyme and its inhibition by caffeine, investigated using fluorimetric method. Archives of Oral Biology 53, 423-428.

37. Grzegorz Mędza, Jacek WIERZCHOWSKI, Borys Kierdaszuk, David Shugar, 2009, Fluorescence emission properties of 8-aza analogues of caffeine, theophylline, and related N-alkyl xanthines. Bioorganic & Medicinal Chemistry 17, 2585-2591.

38. Małgorzata Bogucka, Joanna Giebułtowicz, Katarzyna Zawada, Piotr Wroczyński, Jacek WIERZCHOWSKI, Monika Pietrzak, Piotr Piekarczyk, Katarzyna Romanowska,, 2009, The oxidation status of ALDH3A1 in human saliva and its correlation with antioxidant capacity measured by ORAC method. Acta Pol. Pharm. Drug Res. 66, 477-482.

39. Joanna Giebułtowicz, Renata Wolinowska, Anna Sztybor, Monika Pietrzak, Piotr Wroczyński and Jacek WIERZCHOWSKI, 2009, Salivary Aldehyde Dehydrogenase: Activity Towards Aromatic Aldehydes and Comparison with Recombinant ALDH3A1, Molecules 14,  2363-2372.

40. Joanna Giebułtowicz, Marta Dziadek, Piotr Wroczyński, Katarzyna Woźnicka, Barbara Wojno, Monika Pietrzak and Jacek WIERZCHOWSKI, 2010, Salivary Aldehyde Dehydrogenase: temporal and population variability, correlations with drinking and smoking habits and activity towards aldehydes contained in food, Acta Biochim. Polon. 57, 361-368.

41. Stachelska-Wierzchowska Alicja, Wieczorek Zbigniew J., WIERZCHOWSKI Jacek, 2012, The hydrolysis of 5’-cap dinucleotide analogs, catalyzed by Bi- and Terpyridine complexes of Cu2+ and Zn2+ ions. Nucleosides, Nucleotides & Nucl. Acids 31, ......

 

Reviews:

  1. Wierzchowski J. (2010) Excited-state proton transfer in nucleic acid bases, nucleosides, and their analogues: a mini-review. Current Topics in Biophysics on-line (Poznan, Poland) 33, 9 – 16. (http://www.staff.amu.edu.pl/~ctbo/issue33/v33_9.pdf).


 

 

Prowadzone granty finansowane przez MNiSW:

„Dehydrogenaza aldehydowa w ślinie – pomiar aktywności enzymu i wstępna ocena jego znaczenia dla bezpieczeństwa żywienia” N312 035 31/2169 (umowa wwen. 529-0706.901)

 

Opieka naukowa nad młodymi pracownikami naukowymi:

mgr Grzegorz Mędza, asystent w KFiB od 2006 roku.